ABSTRACT
Objectives: to detect extended spectrum beta lactamase in E. coli and Klebsiella pneumonia in bacterial cultures and its frequency at Lady Reading Hospital, Peshawar
Methodology: this cross sectional analytical study was conducted at LRH between June 2013 to December 2013, a total of 1037 bacterial isolates in-cluding 614 E. coli and 423 of Klebsiella pneumoniae were evaluated. All cases were subjected to double disc diffusion method for ESBL detection using amoxacilln-clavulanic acid and a third generation cephalosporin as all ESBLs are hydrolysed by clavulanic acid. The data were analysed using SPSS-16
Results: patients' mean age was 40 years. Out of 1037 cases, 592 [55%] were males and 445 [45%] were females. Of these, E. Coli were 614 [59.2%] and K. Pneumoniae were 423 [40.8%].Frequency of ESBL positivity in E. coli isolates was 264 [43%] and in Klebsiella pneumonia isolates was 231 [54.6%].Frequency of ESBL in pus was 34.3%[152/395],in urineit was 31.8%[141/368], in blood it was 28.6%[127/233] and in sputum it was 5.1% [23/41].Unit-wise frequency of ESBL was surgical and allied 24.6%[109/283], medical and allied 21.4% [95/241], paediatrics 18.5%[82/203], obstetrics andgynaecology23.2% [103/178] and outpatients 12.1%[54/132]. No significant correlation between ESBL positivity and age, gender, unit or specimen was found
Conclusion: ESBL positive isolates of E. coli and K. pneumoniae account for a very high percentage of hospital-acquired infections. These results should be considered while prescribing penicillins and cephalosporins for treating gram-negative acquired infections